Device

Part:BBa_K4611004:Design

Designed by: Saiyu Luo   Group: iGEM23_CJUH-JLU-China   (2023-10-03)


crRNA full length targeting the miR-21-5p under T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

The crRNA origional sequence is from Leptotrichia wadei. The sequence of T7 promoter is from GenBank: Enterobacteria phage T7, complete genome.This part is improved by CJUH-JLU-China.

References

1.Damalas SG, Batianis C, Martin-Pascual M, de Lorenzo V, Martins Dos Santos VAP. SEVA 3.1: enabling interoperability of DNA assembly among the SEVA, BioBricks and Type IIS restriction enzyme standards. Microb Biotechnol. 2020 Nov;13(6):1793-1806. doi: 10.1111/1751-7915.13609. Epub 2020 Jul 25. PMID: 32710525; PMCID: PMC7533339.

2.https://2022.igem.wiki/thessaloniki-meta/index.html